Differential expression MA plots - deseq2
Date: 02/03/2022
Info
General info:
- Differential expression analysis using deseq2.
- Some values have been rounded
Datasets analysed:
- transcript rnaseq: TRUE
- gene rnaseq: TRUE
Treatment comparisons:
- treatment1 - treatment2
Total number of samples: 4
Number of samples in each treatment group:
- treatment1: 2
- treatment2: 2
MA plots
These plots plot the log2 fold changes versus the mean of normalized counts. These can be used to visualize the differences between treatment group comparisons. In general we would expect the expression of genes/transcripts to remain consistent between treatment groups and so the MA plot should be similar to the shape of a trumpet with most points residing on a y intercept of 0. Differentially expressed genes/transcripts are marked blue.
Genes/transcripts with a p-value smaller than 0.1 and a log fold change greater/smaller than +-log2(1.1) are plotted here.
Blue: Genes/transcripts with an adjusted p-value smaller than 0.1 and a log fold change greater/smaller than +-log2(1.1)
Red: Genes/transcripts with an adjusted p-value smaller than 0.1 and a log fold change greater/smaller than +-log2(1.1)
Yellow: Genes/transcripts with an adjusted p-value smaller than 0.05 and a log fold change greater/smaller than +-log2(1.1)
Green: Genes/transcripts with an adjusted p-value smaller than 0.01 and a log fold change greater/smaller than +-log2(1.1)